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Table 3 Saccharomyces cerevisiae strains used in this study

From: Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation

Strain name Description Source
BY4741 MAT a his 3Δ1 leumet 15Δ ura Euroscarfa
Yeast KO collection MAT a his 3Δ1 leumet 15Δ ura 3Δ ORFΔ::KanMX OpenBiosystemsb
CEN.PK113-7D MATa Euroscarfa
CEN.PK113-1A MATα Euroscarfa
IMK439 MATα ura3Δ::KanMX This study
IMK440 MATa ura3Δ::KanMX This study
Deletion strains* MATa ORFΔ::KanMX This study
Overexpression strains* MATa ORFpr::KanMX-TPI1pr::ORF This study
IMS0344 MATa rpn4-1 rtg1-1 ubr1-1 ssk2-1 This study
IMS0345 MATa/MATα RPN4/rpn4-1 RTG1/rtg1-1 UBR1/ubr1-1 SSK2/ssk2-1 URA3/ura3Δ::KanMX This study
IMS0346 MATα rpn4-1 rtg1-1 ubr1-1 ssk2-1 This study
IMS0347 MATa/MATα RPN4/rpn4-1 RTG1/rtg1-1 UBR1/ubr1-1 SSK2/ssk2-1 URA3/ura3Δ::KanMX This study
IMS0348 MATα rpn4-1 rtg1-1 ubr1-1 ssk2-1 This study
IMS0349 MATa/MATα RPN4/rpn4-1 RTG1/rtg1-1 UBR1/ubr1-1 SSK2/ssk2-1 URA3/ura3Δ::KanMX This study
IMS0350 MATa rpn4-1 rtg1-1 ssk2-1 This study
IMS0351 MATa rpn4-2 rtg1-2 ubr1-2 nma111-2 rpl10-2 sto1-2 sst4-2 This study
IMS0352 MATa/MATα RPN4/rpn4-2 RTG1/rtg1-2 UBR1/ubr1-2 NMA111/nma111-2 RPL10/rpl10-2 STO1/sto1-2 SST4/sst4-2 URA3/ura3Δ::KanMX This study
IMS0353 MATa rpn4-2 rtg1-2 nma111-2 rpl10-2 This study
IMS0354 MATa/MATα RPN4/rpn4-1 RTG1/rtgi-2 NMA111/nma111-2 RPL10/rpl10-2 URA3/ura3Δ::KanMX This study
IMS0355 MATa rpn4-2 rtg1-2 nma111-2 rpl10-2 This study
IMS0356 MATa/MATα RPN4/rpn4-2 RTG1/rtg1-2 NMA111/nma111-2 RPL10/rpl10-2 URA3/ura3Δ::KanMX This study
IMS0357 MATa rpn4-2 rtg1-2 nma111-2 This study
IMI218 MATa RPN4pr::KanMX RPN4pr-rpn4-1 This study
IMI238 MATa RTG1pr::KanMX RTG1pr-rtg1-1 This study
  1. * the complete list of the deletion and overexpression strains is provided in Additional file 1: Table S1. ahttp://web.uni-frankfurt.de/fb15/mikro/euroscarf/, bhttp://www.thermoscientificbio.com/molecular-biology/.