Theoretical glucose yields for 72 hr hydrolysis reactions on assay PCS material. Initial total solids concentration of 5-7% w/w. Enzyme loading of 34 mg EP/g cellulose in assay substrate. Lignin residue added was approximately 95% lignin and ash, and 5% cellulose. “Fraction of lignin residue of total insoluble solids” refers to the percentage of the initial total insoluble solids, which was the lignin residue (total insoluble solids were added lignin residue and unhydrolyzed PCS). Residue was deactivated by heating to 100°C for 10 minutes in a block heater. Values are average of duplicate experiments. Error bars represent ± one standard deviation.