Low-level electrotransformation of C. pasteurianum . A. Colony PCR confirmation of pMTL85141 presence in C. pasteurianum transformants using primers pMTL.seq.S and pMTL.seq.AS. The expected product of 518 bp could only be amplified from E. coli transformed with pMTL85141 and from thiamphenicol-resistant C. pasteurianum colonies, and not from non-recombinant C. pasteurianum. B. XhoI-linearized pMTL85141 plasmid prepared from E. coli DH5α and from a representative transformant of C. pasteurianum showing the expected plasmid size of 2,963 bp. Some undigested vector remains visible in the E. coli preparation.