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Figure 4 | Biotechnology for Biofuels

Figure 4

From: Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum

Figure 4

Investigation of membrane permeabilization on the electrotransformation of C. pasteurianum . Immediately prior to pulse delivery, cell-DNA suspensions were supplemented with 5, 10, or 15% ethanol (EtOH) or 1 or 2% butanol (BuOH). An untreated sample was included as a control. The time constant of each pulse is shown (ο). The sample treated with 2% butanol failed to grow during the allotted 16-hour recovery period following electroporation.

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