Figure 2
From: Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development
Western blot of different transgenic lines constitutively expressing TrCel5A after SDS-PAGE (A) and zymography performed with SDS-PAGE containing 0.15% (w/v) CMC (B). Lanes contain 10 μg of plant total soluble protein. The recombinant enzyme was detected with a polyclonal α-cellulase antibody and an alkaline phosphatase conjugated goat-anti-rabbit secondary antibody. The zymogram control is performed with purified TrCel5A produced in Hansenula polymorpha.