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Table 2 Primers used in this study

From: Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels

Name

Sequence (5′ → 3′)

HMGS-F

GGAATTCCATATG AAACTCTCAACTAAACTTTG

HMGS-R

CACCTCGAG TTATTTTTTAACATCGTAAGATC

tHMGR-F

CATGCCATGG ACCAATTGGTGAAAACTGAAG

tHMGR-R

CGGGATCC TTAGGATTTAATGCAGGTGACG

mvaS-F

CCAGAGCTC AGGAGGTAAAAAAACATGACAATTGGGATTGATAAAATTA

mvaS-R

CAACTGCAG TTAGTTTCGATAAGAGCGAACG

mvaE-F

CATGCCATGG AGGAGGTAAAAAAACATGAAAACAGTAGTTATTATTGATGC

mvaE-R

CGCGGATCC TTATTGTTTTCTTAAATCATTTAAAATAG

IDIKO-F

GTCTTTTGGATCC GTTGTTAGCTC

IDIKO-R

CAAAACTGCAG TTATTCCTTTGGTAGACCAGTC

phoA-F2

CTCAGATCT CCGGACACCAGAAATGCCTGTTC

phoA-R

CACCTCGAG TTATTTCAGCCCCAGAGCGGC

DPP1-F2

CTCAGATCT CCAACCGTTCGAACGTCAGTTTTAC

DPP1-R

CACCTCGAG TTACATACCTTCATCGGACAAAG

LPP1-F2

CTCAGATCT CGAGATATCCCTGGTACCTAGGG

LPP1-R

CACCTCGAG TTACTAAACACTAACCGGTGAAGGAAG

phoE-F

CTAGCCATGG GCATGACAGCCGTTTGTTTAGTAAG

phoE-R

CAGGGATCC TTATTTGATAAAGCCGGATAAGTG

BsNudF-F

CTAGCCATGG GCATGAAATCATTAGAAGAAAAAAC

BsNudF-F

CAGGGATCC TCATTTTTGTGCTTGGAGCG

EcNudF-F

CTAGCCATGG GCATGCTTAAGCCAGACAACCTG

EcNudF-F

CAGGGATCC TTATGCCCACTCATTTTTTAACG

nudC-F

CTAGCCATGG ATCGTATAATTGAAAAATTAG

nudC-R

CAGGGATCC TCACTCATACTCTGCCCGACAC

yggV-F

CTAGCCATGG GCATGCAAAAAGTTGTCCTCGCAAC

yggV-R

CAGGGATCC TTAACCATTACGTAAAGCGTCC

hisl-F

CTAGCCATGG GCATGTTAACAGAACAACAACGTCG

hisl-R

CAGGGATCC TCACTGATGCCGTTTACGCAG

lpxH-F

CTAGCCATGG CGACACTCTTTATTGCAG

lpxH-R

CAGGGATCC TTAAAACGGAAAATGAATCAGCTC

ppa-F

CTAGCCATGG GCATGAGCTTACTCAACGTCCCTG

ppa-R

CAGGGATCC TTATTTATTCTTTGCGCGCTCG

cdh-F

CTAGCCATGG GCATGAAAAAAGCGGGTCTTCT

cdh-R

CAGGGATCC TTAACGCAAAATCTCACACTG

KnudF-F

TCGCTGAAATTCACATTTAATTCACTATTAGTGCCAGGACATTAACAATGCATGGGAATTAGCCATGGTCC

KnudF-R

TCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAATGTAGGCTGGAGCTGCTTCG

KyggV-F

GCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCATGGGAATTAGCCATGGTCC

KyggV-R

TGTGAATGTAGAGACTCAGCGGAGGTAATTTAACCATTACGTAAAGCGTCTGTAGGCTGGAGCTGCTTCG

KnudF-vF

ACCCAGAAAGGCTCAGGCCG

KnudF-vR

ACAGTTTCGCCGGGTGCGTC

KyggV-vF

GACGGCCAGGCCAACAGTCA

KyggV-vR

CGGCCCTGAGCGTAAGCCAC

A110G-F

CTCTTTCGAAATCAAGGAAGGTTGTTACGGAGC

A110G-R

CCTTCCTTGATTTCGAAAGAGCGAGCGAAAG

  1. Underlines indicate restriction enzyme sites.