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Table 1 Strains and plasmids used in this study

From: Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene

Name Relevant characteristics References
Strains   
E.coli BL21(DE3) F-ompT hsdSB (rB-mB-) gal dcm rne131 λ(DE3) Invitrogen
Saccharomyces cerevisiae Type strain ATCC 4040002
YJM26 E.coli BL21(DE3)/pYJM23 This study
YJM27 E.coli BL21(DE3)/pYJM25 This study
YJM28 E.coli BL21(DE3)/pYJM27,pYJM14 This study
YJM29 E.coli BL21(DE3)/pYJM28 This study
Plasmids   
pACYCDuet-1 P15A (pACYC184), Cmr Novagen
pTrcHis2B pBR322 origin, Ampr Invitrogen
pYJM14 pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from Saccharomyces cerevisiae (Yang et al., 2011) [35]
pYJM22 pACYCDuet-1 carrying ispA from E.coli BL21(DE3) This study
pYJM23 pACYCDuet-1 carrying ispA from E.coli BL21(DE3) and Pt30 from Pinus taeda This study
pYJM24 pACYCDuet-1 carrying GPPS2 from Abies grandis This study
pYJM25 pACYCDuet-1 carrying GPPS2 from Abies grandis and Pt30 from Pinus taeda This study
pYJM26 pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis, GPPS2 from Abies grandis This study
pYJM27 pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis, GPPS2 from Abies grandis, Pt30 from Pinus taeda This study
pYJM28 pACYCDuet-1 carrying Pt30 from Pinus taeda This study