Table 1 Strains and plasmids used in this study
From: Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
Name | Relevant characteristics | References |
|---|---|---|
Strains | ||
E.coli BL21(DE3) | F-ompT hsdSB (rB-mB-) gal dcm rne131 λ(DE3) | Invitrogen |
Saccharomyces cerevisiae | Type strain | ATCC 4040002 |
YJM26 | E.coli BL21(DE3)/pYJM23 | This study |
YJM27 | E.coli BL21(DE3)/pYJM25 | This study |
YJM28 | E.coli BL21(DE3)/pYJM27,pYJM14 | This study |
YJM29 | E.coli BL21(DE3)/pYJM28 | This study |
Plasmids | ||
pACYCDuet-1 | P15A (pACYC184), Cmr | Novagen |
pTrcHis2B | pBR322 origin, Ampr | Invitrogen |
pYJM14 | pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from Saccharomyces cerevisiae | (Yang et al., 2011) [35] |
pYJM22 | pACYCDuet-1 carrying ispA from E.coli BL21(DE3) | This study |
pYJM23 | pACYCDuet-1 carrying ispA from E.coli BL21(DE3) and Pt30 from Pinus taeda | This study |
pYJM24 | pACYCDuet-1 carrying GPPS2 from Abies grandis | This study |
pYJM25 | pACYCDuet-1 carrying GPPS2 from Abies grandis and Pt30 from Pinus taeda | This study |
pYJM26 | pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis, GPPS2 from Abies grandis | This study |
pYJM27 | pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis, GPPS2 from Abies grandis, Pt30 from Pinus taeda | This study |
pYJM28 | pACYCDuet-1 carrying Pt30 from Pinus taeda | This study |