Table 1 Oligonucleotides used in this study
Name | Sequence (5′ - 3′) | Usage |
|---|---|---|
act1f | TGAGAGCGGTGGTATCCACG | qPCR |
act1r | GGTACCACCAGACATGACAATGTTG | |
cbh1f | GATGATGACTACGCCAACATGCTG | |
cbh1r | ACGGCACCGGGTGTGG | |
cbh2f | CTATGCCGGACAGTTTGTGGTG | |
cbh2r | GTCAGGCTCAATAACCAGGAGG | |
taqxyn2f | GGTCCAACTCGGGCAACTTT | |
taqxyn2r | CCGAGAAGTTGATGACCTTGTTC | |
taqxyr1f | CCCATTCGGCGGAGGATCAG | |
taqxyr1r | CGAATTCTATACAATGGGCACATGGG | |
sar1fw | TGGATCGTCAACTGGTTCTACGA | |
sar1rev | GCATGTGTAGCAACGTGGTCTTT | |
xyn1f | CAGCTATTCGCCTTCCAACAC | |
xyn1r | CAAAGTTGATGGGAGCAGAAG | |
FT161f | GGAGGCCACTCAATCGTATGA | xyr1 3′-UTR cloning |
FT162r | CGTGCCGCAATCCGGTTGTT | |
FT163f | TGGGTGGTGGTATAGTCTTAAGGGAGGCCACTCAATCGTATGA | |
FT164r | ACGGCCAGTGAATTCTTAATTAACGTGCCGCAATCCGGTTGTT | |
FT165f | GATGTGGCAGCCGGGGAA | xyr1 5′-UTR and coding sequence cloning |
FT166r | TTAGAGGGCCAGACCGGTTC | |
FT167f | ACTCTAGATTAATTAAGATGTGGCAGCCGGGGAA | |
FT168r | GCTTTCGCCACGGAGCTTTAGAGGGCCAGACCGGTTC | |
FT169f | TGCCTGCAGGTCGACTCTAGATTAATTAAGATGTGGCAGCCGGGGAA | |
FT170f | CGGTCTGGCCCTCTAAAGCTCCGTGGCGAAAGCCT | cbh1 3′-UTR cloning |
FT171r | GACGAATGATGGCGGCCGCTTTCCAGGCCGCCAGCTATG | Â |