Analysis of activity of different cassettes introduced into cv. 81V9 T
plants. A. Northern blot analysis of xynA and aadA transcript abundance produced from different CECs. Lanes 1 through 4 represent total plant RNA extracted from T1 transplastomic plants for CEC1- through CEC4-XynA, respectively. Lane 5 represents WT RNA. Equal amounts of RNA were loaded in each lane and confirmed by staining (left panel); the blot hybridized to a xynA-specific probe (middle panel) and aadA-specific probe (right panel) displayed differences in transcript levels and sizes for both genes produced from different CECs. B, C. Immunoblot of XynA (B) and AadA (C) protein product produced from different CECs and assessed in the same leaves sampled for the RNA analyses (A). Each lane represents 0.4 mg of extracted leaf tissue. Lane 5 - WT leaf extract. Known amounts (in ng) of a c-myc-tagged control protein are indicated above the standard curve lanes (B).