Figure 6
Tobacco cv. I64 T 1 transplastomic plant development and spatial expression patterns of different xylanases. A. Phenotypes of T1 transplastomic cv. I64 plants at 40 days post-germination. A slight developmental delay, which was observed to various extent in the transplastomic lines, was completely compensated during further growth as the plants reached maturity, displaying very similar size and flowering time when compared with WT untransformed plants (B, also Table 2). One-meter ruler is pictured on the left for a size reference. C. Western blot-assisted assessment of the spatial accumulation profiles of XynA, Xyn10A and Xyn11B in mature cv. I64 plants. Lanes 1 through 10 represent extracts from 10 leaves (top to bottom), each lane represents extract equivalent to 2.5 mg leaf tissue for XynA and Xyn10A expressed from CEC4; for Xyn10A expressed from CEC5 and Xyn11B expressed from CEC4 and CEC5 each lane represents extract equivalent to 0.1 mg leaf tissue. Known amounts (in ng) of a c-myc-tagged control protein are indicated above the standard curve lanes.