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Table 2 qPCR of differentially expressed genes following N deprivation

From: Molecular and cellular mechanisms of neutral lipid accumulation in diatom following nitrogen deprivation

qPCR

RNA-Seq

Locus tag

Forward (5’-3’)

Reverse (5’-3’)

FC(N/Ctr)

FC Log2(N/Ctr)

Z-test

FDR

54257

agaatcggtgatgcctgttc

atgccgctgctttagtgaat

103.3

5.80

0

0

45012

acgattcggacgaagatcag

ccatgcaacaatcgtagtgg

26.0

5.56

6.71E-14

1.99E-13

27877

acaccaccgacaagaccttc

tccagacagagcgtacaacg

116.6

5.46

0

0

54465

gagcacttcgttctccaagg

gtccagaaagccacagcttc

−17.1

−8.98

0

0

13154

caggaactcgcgaagttagg

gcaagaatggaacccactgt

3.35

7.42

2.46E-5

4.37E-5

45852

aaggccacaatctcatggac

cttttgacggatggcaactt

23.6

7.41

7.47E-9

1.63E-8

51183

ctttacaacgccctgatcgt

ttgctgtcgtggaaagactg

−15.7

−8.65

0

0

9794

gcatattggaggctttggaa

tctgcatcatcatcccgata

1.35

−0.58

7.29E-3

0.010

  1. Each value corresponds to the mean of three biological sample performed in duplicates; β- actin is used as house-keeping gene in real-time quantitative PCR; significant changes are indicated by a p value<0.05 (Z-test).