Figure 5

The β-isopropylmalate dehydrogenase involvement in the glycine degradation pathway. The β-isopropylmalate dehydrogenase activity was tested in a coupled reaction using glyoxylate as substrate instead of β-ethylmalate (not commercial available). The carbons of the molecules were numbered. (A) Reaction catalysed by the β-isopropylmalate dehydrogenase enzyme. (B) Activity of β-isopropylmalate dehydrogenase measured in yeast strain ΔLEU2 (empty column) and overexpressing LEU2 gene (grey column). The data presented here are representative of three independent experiments. p ≤ 0,05 = *; p ≤ 0,01 = **; p ≤ 0,001 = ***; p > 0,05 = n.s.