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Table 1 β-Isopropylmalate dehydrogenase activity measured with assay coupling the reactions catalyzed by Mls1 and Leu2

From: A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae

Gene

β-IPMD Activity (U/mg prot)

MLS1

DAL7

LEU2

 

+

+

++

0.098 ± 0.010

+

+

-

0.023 ± 0.001

+

-

++

0.019 ± 0.002

+

-

-

0.016 ± 0.002

-

+

++

0.021 ± 0.002

-

+

-

0.018 ± 0.002

  1. The symbols “+” indicate the wild type copy or “++” the overexpression of the corresponding gene in the table. The symbol “-” indicate the deletion of the gene. The data presented here are representative of three independent experiments.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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