Table 2 Strains and plasmids used in this work
From: A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae
Parental strain | ||
|---|---|---|
Strain | Genotype | |
CEN.PK 102-5B | MATa, ura3-52, his3-11, leu2-3/112, TRP1, MAL2-8c, SUC2 | |
CEN.PK RWB837 | MATa, pdc1::loxP, pdc5::loxP, pdc6::loxP, ura3-52 | |
BY4741 | MATa, ura3Δ0, leu2Δ0, met15Δ0, his 3Δ1 | |
BY4741ΔMLS1 | MATa; his3 Δ1; leu2 Δ0; met15 Δ0; ura3 Δ0; YNL117w::kanMX4 | |
BY4741ΔDAL7 | MATa; his3 Δ1; leu2 Δ0; met15 Δ0; ura3 Δ0; YIR031c::kanMX4 | |
Plasmids | ||
Expression plasmid | Segregation | Markers, genes |
pYX212 | multicopy (2μ derived) | URA3 |
pYX212goxB opt | multicopy (2μ derived) | URA3, optimised goxB |
pYX022 | integrative | HIS3 |
pYX242 | multicopy (2μ derived) | LEU2 |
Transformed strain | ||
Strain | Plasmid | Obtained strain |
CEN.PK 102-5B | pYX212, pYX022, pYX242 | CEN.PKc |
BY4741 | pYX212, pYX022, pYX242 | BY4741c |
BY4741 | pYX212goxB opt, pYX022, pYX242 | BY4741c goxB opt |
BY4741ΔMLS1 | pYX212, pYX022, pYX242 | BY4741ΔMLS1c |
BY4741ΔMLS1 | pYX212goxB opt, pYX022, pYX242 | BY4741ΔMLS1c goxB opt |
BY4741ΔDAL7 | pYX212, pYX022, pYX242 | BY4741ΔDAL7c |