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Table 1 Plasmids constructed and used in this study

From: Engineering cyanobacteria to improve photosynthetic production of alka(e)nes

Plasmid

Relevant characteristicsa, b

Reference

pFQ9R

Apr Sper, pKW1188sl derivative containing Omega, Trbc terminator, Prbc promoter

[5]

pXT37b

Apr, Sper, pUC9' derivative containing upstream and downstream fragments of slr1609, Omega and PpetE promoter.

[5]

pXT119

Apr, pMD18-T derivative containing upstream and downstream fragments of slr1556

This study

pLX1

Apr Sper, pFQ9R derivative containing sll0208 gene, Prbc promoter

This study

pLX3

Apr Sper, pFQ9R derivative containing sll0208 and sll0209 gene, Prbc promoter

This study

pLX4

Apr Sper, pXT37b derivative containing sll0209 gene, PpetE promoter

This study

pLX5

Apr Sper, pFQ9R derivative containing orf1593 and orf1594 gene, Prbc promoter

This study

pLX6

Apr Sper, pFQ9R derivative containing npun1710 and npun 1711 gene, Prbc promoter

This study

pLX9

Apr Sper, pFQ9R derivative containing orf1594 and npun 1711 gene, Prbc promoter

This study

pLX13

Apr, Kanr, pXT119 derivative containing sll0208 and sll0209 gene, CK2, and Prbc promoter.

This study

pLX14

Apr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter.

This study

pLX27

Apr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter, Trbc terminator.

This study

pLX28

Apr, Kanr, pGEM-T Easy derivative containing sll0208 and sll0209 gene, Prbc promoter, Trbc terminator, CK2.

This study

pLX59

Apr Sper, pFQ9R derivative containing two fragments of Prbc promoter , sll0208 and sll0209 gene.

This study

  1. a Ap, Ampicillin. Spe, Spectinomycin. Kan, kanamycin.
  2. bCK2 for kanamycin antibiotic resistance gene and Omega for spectinomycin antibiotic resistance gene.