Skip to main content

Table 2 Synechocystis strains constructed and used in this study

From: Engineering cyanobacteria to improve photosynthetic production of alka(e)nes

Strain

Genotypea,

Reference

6803yu

Synechocystis sp. PCC6803 Wild-type, Glucose-tolerance

Prof. Xudong Xu

XT203

slr1993∷CK2 sacB

This lab

GQ4

psbA2∷CK2 PpsbA2slr1609

This lab

GQ10

slr0168∷Omega Prbcsll1969 Trbc

This lab

Syn-20ACC

slr0168::Omega P rbcl accBCDA (PCC6803) Trbc

[5]

LX31

slr0168∷Omega Prbcsll0208 Trbc

This study

LX32

slr0168∷Omega Prbcsll0208&sll0209 Trbc

This study

LX33

slr0168∷Omega PpetEsll0209

This study

LX34

slr0168∷Omega Prbcorf1593&orf1594 Trbc

This study

LX35

slr0168∷Omega npun1710&npun 1711 Trbc

This study

LX38

slr0168∷Omega Prbcsll0208&sll0209Trbc, psbA2∷CK2 PpsbA2slr1609

This study

LX39

slr0168∷Omega orf1594&npun 1711 Trbc

This study

LX40

slr0168∷Omega Prbcsll0208&sll0209 Trbc, slr1993∷CK2 sacB

This study

LX55

slr1556∷CK2 Prbcsll0208&sll0209 Trbc

This study

LX56

slr0168∷Omega Prbcsll0208&sll0209 Trbc, slr1556∷CK2 Prbcsll0208&sll0209 Trbc

This study

LX57

slr1556∷CK2 Prbcsll0208&sll0209 Trbc, slr0168::Omega Prbcl accBCDA (PCC6803) Trbc

This study

LX58

slr1556∷CK2 Prbcsll0208&sll0209 Trbc, slr0168∷Omega Prbcsll1969 Trbc

This study

LX70

slr0168∷Omega Prbcsll0208&sll0209 Prbcsll0208&sll0209

This study

  1. aPpetE, 0.4 kb DNA fragment containing the promoter of petE gene. Prbc, 0.3 kb DNA fragment containing the promoter of rbc operon. P rbcl , 1.3 kb DNA fragment containing the promoter of rbc operon. Trbc, 0.2 kb downstream DNA fragment of rbcS gene. PpsbA2, 1.5 kb DNA fragment containing the promoter of psbA2 gene. All promoters and terminators mentioned here are from Synechocystis sp. PCC 6803.