Cofactor engineering through heterologous expression of an NADH oxidase and its impact on metabolic flux redistribution in Klebsiella pneumoniae

Table 1 Effect of the NAD ⁺ regeneration system introduction on the metabolic flux distribution in Klebsiella pneumoniae (unit: mol/mol glucose)

Metabolites^a	K. pneumoniae CICC10011	K. pneumoniae XZF-308	Fluxes redistributed by introducing the NAD⁺ regeneration system
Acetoin	0.056	0.162	189.29%
2,3-Butanediol	0.400	0.398	−0.5% ^b
Acetic acid	0.093	0.102	9.5%
Lactic acid	0.033	0.029	−12.2%^b
Ethanol	0.055	0.048	−12.9% ^b

^a Acetoin is an NAD⁺ dependent metabolite, 2,3-butanediol, lactic acid, and ethanol are NADH dependent metabolites, while acetic acid is not dependent of NADH or NAD⁺.
^b Negative standard means a decrease in glucose flux to 2,3-butanediol, lactic acid, and ethanol.

ISSN: 2731-3654