Temperature induced inactivation of Dieselzymes. (A) Inactivation as a function of temperature. Residual activity was measured after incubation for 1 hour at various temperatures. (B) Time course of thermal inactivation at 50°C. Results are the average of 3 independent experiments. Error-bars are omitted but are less than 5% in all cases. Lines are shown for clarity and represent the best-fit to the data points. WT PML (black circles), BCL (red open circles), Dieselzyme 1 (blue squares), Dieselzyme 2 (blue diamond), Dieselzyme 3 (green triangle), and Dieselzyme 4 (orange inverted triangle).