Operational stability of covalently immobilized Wild-Type PML, Dieselzyme 4, and BCL. For each, the same amount of initial activity was added such that >40% conversion was seen over the first four hours: 0.25 g immobilized WT PML; 0.25 g immobilized Dieselzyme 4, or 0.1 g BCL. For each round, the reaction conditions were: 1.5 mL canola oil, 312.5 μL methanol (5:1 MeOH:oil), 312.5 μL 0.1 M sodium phosphate pH 7.0 (20% v/v with oil); temperature 25°C; 180 rev/min. During cycles 1, 4, and 7, 100 μL samples were taken at 0, 2, and 4 hours to monitor rate of reaction. After each 20 hour cycle, beads were washed with buffer followed by hexanes prior to addition of fresh substrates and buffer. The data are the average of two experiments.