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Table 1 List of mutations in engineered Dieselzymes

From: Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution


Mutations present

Dieselzyme 1


Dieselzyme 2


Dieselzyme 3


Dieselzyme 4


  1. Mutations in bold indicate the new mutations that were identified in each round of directed evolution. The additive effect of mutations was analyzed by combining mutations through site-directed mutagenesis. Only the final mutant from each round is shown. * - Construct used as parent for the initial round of directed evolution. # - N17S mutation arose spontaneously while combining the G202E, G266S, and D270N mutations.