Figure 4

Partial HSQC spectra showing the side-chain C-H correlations from the three main linkages (β-O-4-, β -5-, and β-β) in lignins and presence of wall-associated fucose. (a) Partial HSQC spectra of isolated lignins. The carbon positions in aromatic ring and side-chain are labeled on the molecular structures of the A, B and C types of linkages. The insert table shows the relative content of different linkage types. (b) Partial 2D HSQC NMR analysis of gel-state CWR showing increased wall-associated fucose in PvMYB-OX. Red, control 2A. Blue, PvMYB4-OX.