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Table 1 Metabolite concentrations (ng/ml; sorbitol equivalents) of methanol extractives by GC-MS

From: Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production

Metabolite

MYB4-OX

Control

MYB4-OX/Control

P-value

Classification

16.07 375 583 513 411 427 204

6

1

8.66

0.000

glucoside

16.23 488 327 265 syringyl lignan

4

1

6.33

0.018

lignan

15.97 583 375 285

70

12

5.98

0.001

N/A

11.01 450 217 sugar

45

11

4.29

0.030

modified sugar

9.96 281 383 354

289

71

4.07

0.022

phenolic

10.27 328 343 284 254

999

245

4.08

0.005

N/A

raffinose

933

267

3.49

0.022

sugar - trisaccharide

galactose

1193

345

3.46

0.056

sugar - monosaccharide

19.47 496 481 209 lignan

36

11

3.31

0.050

lignan

α-tocopherol

18

6

3.20

0.060

vitamin

γ-tocopherol

8

3

2.73

0.018

vitamin

13.93 375 292 305 275 uronic acid

176

69

2.54

0.002

sugar acid conjugate

dehydroabietic acid

76

32

2.36

0.048

resin acid

19.09 483 498 lignan

11

5

2.27

0.047

lignan

tryptophan

264

120

2.21

0.036

amino acid

16.11 368 600 585 353 255

11

5

2.18

0.001

N/A

fructose

8160

3897

2.09

0.083

sugar - monosaccharide

bornesitol

2914

1403

2.08

0.000

cyclitol

glutamine

605

315

1.92

0.081

amino acid

tyrosine

234

123

1.91

0.058

amino acid

α-linolenic acid

1132

674

1.68

0.060

fatty acid

alanine

3061

1835

1.67

0.002

amino acid

glucose

6307

3799

1.66

0.040

sugar - monosaccharide

16.85 caffeic acid conjugate

17

11

1.59

0.031

phenylpropanoid

dodecanoic acid

134

88

1.53

0.090

fatty acid

3-O-caffeoylquinic acid

414

297

1.39

0.146

phenylpropanoid

5-O-caffeoylquinic acid

24

17

1.37

0.073

phenylpropanoid

caffeic acid

42

30

1.37

0.040

phenylpropanoid

GABA (γ-aminobutyric acid)

7451

5457

1.37

0.050

amino acid

shikimic acid

6025

4626

1.30

0.423

organic acid

campesterol

54

43

1.26

0.078

sterol

4-O-caffeoylquinic acid

28

23

1.23

0.405

phenylpropanoid

quinic acid

2716

2670

1.02

0.932

organic acid

sinapic acid

4

6

0.78

0.075

phenylpropanoid

5-O-feruloylquinic acid

134

185

0.72

0.050

phenylpropanoid

19.14 572 498 483

2

2

0.70

0.001

N/A

5-hydroxyconiferyl alcohol

1

1

0.68

0.001

phenylpropanoid

ferulic acid

26

42

0.61

0.001

phenylpropanoid

sinapyl alcohol

6

10

0.58

0.004

phenylpropanoid

sucrose

3600

6212

0.58

0.226

sugar - disaccharide

4-O-feruloylquinic acid

98

173

0.57

0.013

phenylpropanoid

12.88 553 463 373 283

27

49

0.56

0.001

N/A

coniferaldehyde

1

1

0.53

0.006

phenylpropanoid

p-coumaric acid

102

236

0.43

0.000

phenylpropanoid

3-O-feruloylquinic acid

171

413

0.41

0.007

phenylpropanoid

16.32 327 syringyl lignan

227

562

0.41

0.000

lignan

coniferyl alcohol

4

12

0.36

0.000

phenylpropanoid

16.11 327 297 syringyl lignan

23

66

0.35

0.002

lignan

16.76 354 482 439 323 297 lignan

1

3

0.32

0.000

lignan

syringin

3

10

0.31

0.000

phenylpropanoid

16.82 354 456 203 188

0

1

0.30

0.002

N/A

16.06 297 guaiacyl lignan

644

2184

0.30

0.000

lignan

9.99 275

1

4

0.20

0.000

N/A

p-hydroxybenzaldehyde

4

23

0.18

0.000

phenylpropanoid

guaiacylglycerol

45

287

0.16

0.000

phenylpropanoid

15.84 412 323 297 209 lignan

29

207

0.14

0.000

lignan

15.12 518 shikimic acid conjugate

0

2

0.13

0.001

organic acid

16.62 486 576 546 456 209 lignan

9

72

0.13

0.000

lignan

  1. Unidentified and tentatively identified metabolites are designated by retention time (RT; min) and key mass-to-charge (m/z) ratios, with the first designated m/z extracted for relative quantification.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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