Figure 1

Component, photosynthesis and respiration of nutrient starvation treated L. punctata and activity of ADP-glucose pyrophosphorylase. Fronds were collected at different time point and used for fresh weight, dry weight, protein and starch percentage analysis, and enzymatic activity assay for ADP-glucose pyrophosphorylase (EC: 2.7.7.27; AGP) respectively. The protein and starch percentage were calculated basing on dry weight. Activities of AGP were measured following the introduction of Nakamura, Y. et al. [52]. Photosynthesis rate and respiration rate measured at 0 h time point were defined as 100%. For each time point, three culture flasks were chose as replicates for these analyses. A: fresh weight and dry weight correspond to left Y-axis, protein content and starch content correspond to right Y-axis. B: AGP activities correspond to left Y-axis, photosynthesis and respiration correspond to right Y-axis.