Figure 1

Schematic view of the mixture design used in the study. The effect of the feed substrate composition (in fed-batch after cell growth on glucose) was investigated with a 3-factor mixture design which can be represented in a triangle. The studied domain (in green) is defined by the factors ranges: inducer content from 8 to 100% (in red), xylose content from 0 et 92% (in blue), glucose content from 0 to 92% (in brown). For every point in the triangle, the sum of inducer, xylose et glucose contents makes 100%. Circles represent experimental points: mixtures tested in fed-flask protocol to create the models (in orange and purple), mixtures tested in bioreactor to validate the models for lactose inducer (in purple), and central point used to assess protocol reproducibility (in black). This mixture design was repeated twice: once for lactose inducer and once for cellobiose inducer.