Figure 2

Monitoring examples for fed-flask and bioreactor cultivations. pH and concentrations were monitored during fed-flask cultivation (A) and bioreactor cultivation (B) during growth phase with excess glucose (time before 0 h) then during protein production phase under carbon flux limitation (time after 0 h) by feeding with a sugar mixture solution composed of 50% lactose, 12.5% xylose and 37.5% glucose. In fed-flask protocol pH was stabilized owing to a stoichiometric mix of ammonia in the feed, whereas in bioreactor pH was controlled with ammonia pulses.