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Table 4 Enzymatic hydrolysis of alkaline pretreated corn stover a

From: Understanding of alkaline pretreatment parameters for corn stover enzymatic saccharification

Temp (°C)

NaOH loading (g NaOH/g corn stover)

Time (mins)

Hydrolysis yieldb

Pretreatment and hydrolysis yieldc

Enzyme accessibilityd

Glucan

Xylan

Glucan

Xylan

Glucan

Xylan

74

0.052

102

47.35%

45.56%

45.53%

42.32%

56.79%

55.30%

95

0.100

75

82.31%

75.67%

76.75%

58.67%

88.19%

79.84%

130

0.070

75

59.83%

67.21%

57.28%

52.16%

88.42%

79.87%

95

0.070

120

69.63%

70.47%

67.17%

60.17%

83.98%

77.39%

116

0.088

102

82.63%

76.62%

78.66%

59.51%

94.12%

84.55%

74

0.052

48

49.29%

42.53%

47.65%

40.27%

64.21%

58.07%

95 d

0.070

75

68.42%

72.94%

66.81%

61.59%

89.75%

80.58%

116

0.088

48

82.32%

79.22%

79.76%

63.32%

91.42%

82.03%

95

0.040

75

47.18%

38.04%

46.09%

37.14%

59.40%

50.27%

60

0.070

75

55.72%

53.82%

54.35%

49.22%

66.25%

62.45%

74

0.088

102

75.71%

73.24%

72.55%

60.11%

90.89%

80.43%

116

0.052

102

61.11%

61.76%

58.49%

51.96%

95.57%

83.29%

95

0.070

30

68.01%

64.40%

65.59%

56.46%

86.79%

76.17%

116

0.052

48

55.42%

60.37%

53.45%

53.20%

80.88%

71.78%

74

0.088

48

70.54%

72.13%

68.51%

59.43%

88.90%

80.73%

95 d

0.070

75

68.71%

73.00%

66.99%

61.95%

89.95%

81.20%

  1. a Values are expressed as averages of two replicate samples. Coefficient of variation (CV) is below 2.5%.b Glucose and xylose yield was calculated based on glucan and xylan content in pretreated and washed corn stover. c glucose and xylose yield was calculated based on glucan and xylan content in raw corn stover. d Glucan (or xylan) enzyme accessibility was defined as the fraction of glucan (or xylan) in biomass that can be converted to monomeric sugars by enzyme activities. They were obtained by measuring glucan or xylan conversions at an extremely high enzyme dosage (50 mg Cellic® CTec2/g glucan).