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Table 4 Enzymatic hydrolysis of alkaline pretreated corn stover a

From: Understanding of alkaline pretreatment parameters for corn stover enzymatic saccharification

Temp (°C) NaOH loading (g NaOH/g corn stover) Time (mins) Hydrolysis yieldb Pretreatment and hydrolysis yieldc Enzyme accessibilityd
Glucan Xylan Glucan Xylan Glucan Xylan
74 0.052 102 47.35% 45.56% 45.53% 42.32% 56.79% 55.30%
95 0.100 75 82.31% 75.67% 76.75% 58.67% 88.19% 79.84%
130 0.070 75 59.83% 67.21% 57.28% 52.16% 88.42% 79.87%
95 0.070 120 69.63% 70.47% 67.17% 60.17% 83.98% 77.39%
116 0.088 102 82.63% 76.62% 78.66% 59.51% 94.12% 84.55%
74 0.052 48 49.29% 42.53% 47.65% 40.27% 64.21% 58.07%
95 d 0.070 75 68.42% 72.94% 66.81% 61.59% 89.75% 80.58%
116 0.088 48 82.32% 79.22% 79.76% 63.32% 91.42% 82.03%
95 0.040 75 47.18% 38.04% 46.09% 37.14% 59.40% 50.27%
60 0.070 75 55.72% 53.82% 54.35% 49.22% 66.25% 62.45%
74 0.088 102 75.71% 73.24% 72.55% 60.11% 90.89% 80.43%
116 0.052 102 61.11% 61.76% 58.49% 51.96% 95.57% 83.29%
95 0.070 30 68.01% 64.40% 65.59% 56.46% 86.79% 76.17%
116 0.052 48 55.42% 60.37% 53.45% 53.20% 80.88% 71.78%
74 0.088 48 70.54% 72.13% 68.51% 59.43% 88.90% 80.73%
95 d 0.070 75 68.71% 73.00% 66.99% 61.95% 89.95% 81.20%
  1. a Values are expressed as averages of two replicate samples. Coefficient of variation (CV) is below 2.5%.b Glucose and xylose yield was calculated based on glucan and xylan content in pretreated and washed corn stover. c glucose and xylose yield was calculated based on glucan and xylan content in raw corn stover. d Glucan (or xylan) enzyme accessibility was defined as the fraction of glucan (or xylan) in biomass that can be converted to monomeric sugars by enzyme activities. They were obtained by measuring glucan or xylan conversions at an extremely high enzyme dosage (50 mg Cellic® CTec2/g glucan).