Figure 4From: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stoverThe effect of a heat treatment on the sensitivity of ELISA for pure Cel7A (A) and Cel6A (B) and Cel7B (C). Heated enzyme samples were boiled in Na-acetate buffer pH 5.0 at 100°C for 10 minutes and then serially diluted in PBS (□). Non-heated samples were directly diluted in PBS (◊).Back to article page