Figure 8From: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stoverAdsorption profiles of Cel7A (A), Cel6A (B), and Cel7B (C) during hydrolysis of SPCS as determined by a double-antibody sandwich ELISA. Samples for Cel7A ELISA were obtained by hydrolyzing SPCS at 2% substrate consistency in 0.05 M Na-acetate buffer pH 5.0 with 20 FPU/ g cellulose Accellerase 1000. Samples for Cel6A and Cel7B ELISA were obtained from SPCS hydrolysis using 20 FPU/ g cellulose Celluclast and 40 CBU/ g cellulose β-glucosidase.Back to article page