Skip to main content
Figure 8 | Biotechnology for Biofuels

Figure 8

From: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stover

Figure 8

Adsorption profiles of Cel7A (A), Cel6A (B), and Cel7B (C) during hydrolysis of SPCS as determined by a double-antibody sandwich ELISA. Samples for Cel7A ELISA were obtained by hydrolyzing SPCS at 2% substrate consistency in 0.05 M Na-acetate buffer pH 5.0 with 20 FPU/ g cellulose Accellerase 1000. Samples for Cel6A and Cel7B ELISA were obtained from SPCS hydrolysis using 20 FPU/ g cellulose Celluclast and 40 CBU/ g cellulose β-glucosidase.

Back to article page