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Table 1 Rates of n- octane and α-pinene efflux from JA300A expressing AcrB variants

From: Directed evolution of an E. coli inner membrane transporter for improved efflux of biofuel molecules

Hydrocarbons AcrB variants Hydrocarbon release rate constant/min-1 AcrB hydrocarbon efflux rate constanta/min-1 Relative AcrB efflux rate
n-octane none 0.227 ± 0.007 - -
  Wild-type 0.312 ± 0.010 0.085 1.00
  1G1 0.352 ± 0.011 0.125 1.47
  1G2 0.349 ± 0.009 0.122 1.44
  N189H 0.318 ± 0.018 0.091 1.07
  L357Q 0.290 ± 0.011 0.063 0.74
  T678S 0.318 ± 0.025 0.091 1.07
  T678A 0.334 ± 0.015 0.107 1.26
  Q737L 0.355 ± 0.023 0.128 1.51
  M844L 0.325 ± 0.010 0.098 1.15
  M844A 0.339 ± 0.015 0.112 1.31
  M987T 0.292 ± 0.019 0.065 0.77
  V1028M 0.304 ± 0.008 0.077 0.91
α-pinene none 0.282 ± 0.018 - -
  Wild-type 0.297 ± 0.014 0.015 1.00
  1G1 0.342 ± 0.015 0.060 4.00
  1G2 0.328 ± 0.018 0.046 3.07
  N189H 0.322 ± 0.012 0.040 2.69
  L357Q 0.296 ± 0.009 0.014 0.96
  T678S 0.319 ± 0.008 0.037 2.44
  T678A 0.330 ± 0.012 0.048 3.20
  Q737L 0.340 ± 0.008 0.058 3.89
  M844L 0.318 ± 0.008 0.036 2.41
  M844A 0.327 ± 0.019 0.045 3.00
  M987T 0.293 ± 0.005 0.011 0.75
  V1028M 0.297 ± 0.005 0.015 0.97
  1. a Calculated by taking the difference between the hydrocarbon release rate constants of JA300A expressing no AcrB and JA300A expressing variants of AcrB.