Table 2 Strains and plasmids used in this study

Caldicellulosiruptor

C. bescii DSM6725

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C.hydrothermalis DSM18901

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C. kristjansonii DSM12137

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C. saccharolyticus DSM8903

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C. obsidiansis ATCC BAA-2073

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C. lactoaceticus DSM9545

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

C. kronotskyensis DSM12137

Wild type / (ura⁺/5-FOA^S)

DSMZ¹

JWCB005

C. bescii ΔpyrFA / (ura^-/5-FOA^R)

[13]

JWCB018

C. bescii ΔpyrFA ΔcbeI / (ura^-/5-FOA^R)

This study

JWCH003

C. hydrothermalis IScahyI insertion mutation in pryF gene / (ura^-/5-FOA^R)

[13, 36]

JWCH005

JWCH003 transformed with M.CbeI methylated pDCW89 / (ura⁺/5-FOA^S)

[13]

Escherichia coli

JW291

DH5α containing pDCW88 (Apramycin^R)

This study

JW292

DH5α containing pDCW89 (Apramycin^R)

[13]

Plasmids

pDCW88

cbeI kcock-out vector (Apramycin^R)

This study

pDCW89

E. coli/Caldicellulosiruptor species shuttle vector (Apramycin^R)

[13]