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Table 4 Microorganisms used in this study

From: Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24

Strain Genotype (description) Reference
V601 Bacteroides uniformis This work
V923 Bacteroides distasonis This work
V975 Bacteroides ovatus This work
TC2-24 Prevotella ruminicola (NRRL # B-50773) This work
TC27 Prevotella ruminicola This work
D31d Prevotella ruminicola This work
20-63 Prevotella ruminicola This work
20-78 Prevotella ruminicola This work
E40a Prevotella ruminicola This work
E42g Prevotella ruminicola This work
H15a Prevotella ruminicola This work
H2b Prevotella ruminicola This work
118b Prevotella ruminicola This work
20-92A Prevotella ruminicola This work
CEN.PK2-1C S. cerevisiae MAT a ura3-52 trp1-289 leu2-3,112 his3 Δ1 MAL2-8CSUC2 Euroscarf
YRH400 D5A + integrated (KanMX4; P PGK1 -XYL1-T PGK1 ; P ADH1 -XYL2-T ADH1 ; P HXT7 -XKS1-T HXT7 ) [27]
YRH561 CEN.PK2-1C [pRS414, pRS416] (control strain with empty vectors) This work
YRH562 CEN.PK2-1C [pRH195, pRH218] (low copy XKS1, high copy Piromyces XI) This work
YRH563 CEN.PK2-1C [pRH195, pRH351] (low copy XKS1, high copy B. uniformis XI) This work
YRH564 CEN.PK2-1C [pRH195, pRH352] (low copy XKS1, high copy B. distasonis XI) This work
YRH565 CEN.PK2-1C [pRH195, pRH353] (low copy XKS1, high copy B. ovatus XI) This work
YRH587 CEN.PK2-1C [pRH195, pRH367] (low copy XKS1, high copy P. ruminicola XI) This work
YRH592 CEN.PK2-1C [pRH351, pRH369] (low copy B. uniformis XK, high copy B. uniformis XI) This work
YRH628 CEN.PK2-1C [pRH384] (no XKS1, high copy P. ruminicola XIa) This work
YRH629 CEN.PK2-1C [pRH195, pRH384] (low copy XKS1, high copy P. ruminicola XIa) This work
YRH630 CEN.PK2-1C [pRH385, pRH218] (low copy P. ruminicola XKa, high copy Piromyces XI) This work
YRH631 CEN.PK2-1C [pRH385, pRH384] (low copy P. ruminicola XKa, high copy P. ruminicola XIa) This work
YRH1114 YRH631 adapted for improved D-xylose fermentation This work
YRH1136 YRH1114 with XI and XK vectors (pRH384 and pRH385) evicted This work
YRH1137 YRH114 with XI vector (pRH384) evicted, maintains the P. ruminicola XKa [pRH385] This work
YRH1138 YRH114 with XK vector (pRH385) evicted, maintains the P. ruminicola XIa [pRH384] This work
  1. a codon adapted for expression in S. cerevisiae.