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Table 5 Plasmids used in this study

From: Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24

Plasmid Description Reference
pRS414 pBluescript II SK+, TRP1, CEN6, ARSH4 [39]
pRS416 pBluescript II SK+, URA3, CEN6, ARSH4 [39]
pRS426 pBluescript II SK+, URA3, 2μ origin [39]
pJ201 Gene synthesis vector (DNA2.0)
pRH164 pRS414 + P HXT7 -MCS-T HXT7 [27]
pRH167 pRS426 + P HXT7 -MCS-T HXT7 [27]
pRH195 pRS414 + P HXT7 -S. cerevisiae XKS1-T HXT7 [27]
pRH218 pRS426 + P HXT7 -Piromyces sp. E2 XI-T HXT7 [40]
pRH325 pCR2.1 TOPO + Bacteroides uniformis XI This work
pRH326 pCR2.1 TOPO + Bacteroides distasonis XI This work
pRH327 pCR2.1 TOPO + Bacteroides ovatus XI This work
pRH351 pRS426 + P HXT7 -Bacteroides uniformis XI-T HXT7 This work
pRH352 pRS426 + P HXT7 -Bacteroides distasonis XI-T HXT7 This work
pRH353 pRS426 + P HXT7 -Bacteroides ovatus XI-T HXT7 This work
pRH357 pCR2.1 TOPO + Prevotella ruminicola strain TC2-24 XI This work
pRH360 pCR2.1 TOPO + Bacteroides uniformis xylB (D-xylulokinase) This work
pRH367 pRS426 + P HXT7 -Prevotella ruminicola XI-T HXT7 This work
pRH369 pRS426 + P HXT7 -Bacteroides uniformis xylB-T HXT7 This work
pRH379 pJ201 + Prevotella ruminicola TC2-24 codon-adapted XI This work
pRH380 pJ201 + Prevotella ruminicola 23 codon-adapted XK This work
pRH384 pRS426 + P HXT7 -P. ruminicola TC2-24 codon-adapted XI -T HXT7 This work
pRH385 pRS414 + P HXT7 -P. ruminicola 23 codon-adapted XK -T HXT7 This work
pRH544 pRS414 + P HXT7 -P. ruminicola 23 codon-adapted XK -T HXT7 rescued from YRH1114 This work
pRH545 pRS426 + P HXT7 -P ruminicola TC2-24 codon-adapted XI -T HXT7 rescued from YRH1114 This work
  1. For all plasmids listed using the HXT7 promoter, P HXT7 is the truncated version of the HXT7 promoter for constitutive expression.