Figure 2

Representative electron flow in conversion of fatty aldehyde into alk(a/e)ne with the reconstituted ADO/Fd/FNR system. Reducing equivalents from NADPH are transferred from FNR to Fd and then to ADO. The crystal structures of related ADO (PDB ID: 2OC5, 60% sequence identity to ADO from PCC7942) from Prochlorococcus marinus MIT9313, Fd (PDB ID: 1QT9, 82% sequence identity to Fd from PCC7942) and FNR (PDB ID: 1QUE, 67% sequence identity to FNR from PCC7942) from Anabaena sp. PCC7119 were used to demonstrate electron transfer in the reconstituted ADO/Fd/FNR system.