Glycerol and ethanol yield (on glucose) in parental strains, hybrid diploid and segregants. (a) Glycerol and ethanol yield (on glucose) in the parental strains, 26B (■) and ER7A (▲), the hybrid diploid strain 26B/ER7A (●) and in segregants of 26B/ER7A (○). For screening purposes, one fermentation was carried out for each strain in 100 ml YP with 10% glucose. Glycerol and ethanol yields of all segregants, ER7A and the diploid 26B/ER7A were related to the yield of 26B, which was set as 100%. (b,c) Distribution of the glycerol and ethanol yield (on glucose) in the unselected (b) and selected (c) segregant pool of 26B/ER7A. The criteria for selection of “low glycerol” segregants (<120% glycerol yield, >99% ethanol yield) are indicated with stippled lines. The values of the 22 selected segregants are the average of three replicates. These segregants were used for pooled-segregant whole-genome sequence analysis. The glycerol and ethanol yield of the parental strains, 26B and ER7A, and diploid 26B/ER7A are indicated as in (a).