Figure 2From: Identification of multiple interacting alleles conferring low glycerol and high ethanol yield in Saccharomyces cerevisiae ethanolic fermentationGlycerol and ethanol yield (on glucose) in parental strains, hybrid diploid and segregants. (a) Glycerol and ethanol yield (on glucose) in the parental strains, 26B (■) and ER7A (▲), the hybrid diploid strain 26B/ER7A (●) and in segregants of 26B/ER7A (○). For screening purposes, one fermentation was carried out for each strain in 100 ml YP with 10% glucose. Glycerol and ethanol yields of all segregants, ER7A and the diploid 26B/ER7A were related to the yield of 26B, which was set as 100%. (b,c) Distribution of the glycerol and ethanol yield (on glucose) in the unselected (b) and selected (c) segregant pool of 26B/ER7A. The criteria for selection of “low glycerol” segregants (<120% glycerol yield, >99% ethanol yield) are indicated with stippled lines. The values of the 22 selected segregants are the average of three replicates. These segregants were used for pooled-segregant whole-genome sequence analysis. The glycerol and ethanol yield of the parental strains, 26B and ER7A, and diploid 26B/ER7A are indicated as in (a).Back to article page