Reciprocal hemizygosity analysis (RHA). RHA for the candidate genes, SMP1 (chr. II), GPD1 (chr. IV), and HOT1 (chr. XIII) to evaluate them as causative genes in the QTLs. For RHA, diploid strains were constructed with either the deletion of the ER7A allele or the deletion of the 26B allele. Glycerol and ethanol yield (on glucose) of the two hemizygous diploid strains were related to the parental strain 26B. The Student t-test was used to confirm significant differences in glycerol and ethanol yield for the two diploids and is indicated with *. Each strain construct was tested in triplicate.