Figure 6

Expression of gpd1^L164P-CBS4C and GPD1 -ER7A in segregant 26B, ER7A, the diploid 26B/ER7A and BY4742. Glycerol and ethanol yield (on glucose) in the gpd1Δ strains, 26B, ER7A, 26B/ER7A and BY4742, harboring the plasmids YCplac33, YCplac33 GPD1-ER7A, and YCplac33 gpd1^L164P-CBS4C. Fermentations were carried out in 100 ml YP 10% glucose. Each strain construct was tested in triplicate. Glycerol and ethanol yield of the strains were related to the yield of 26B, which was set at 100%. In the BY4742 and ER7A backgrounds, which lack the smp1^R110Q,P269Q and hot1^P107S,H274Y alleles, the gpd1^L164P allele clearly reduced glycerol yield and concomitantly increased ethanol yield compared to the wild type GPD1 allele. In the strains 26B and 26B/ER7A, which contain the smp1^R110Q,P269Q and hot1^P107S,H274Y alleles, the gpd1^L164P allele resulted in a similar glycerol yield as the wild type GPD1 allele.