Distribution of the gpd1L164P, hot1P107S,H274Yand smp1R110Q,P269Qalleles in the selected low glycerol/high ethanol segregants. (a) Glycerol and ethanol yield (on glucose) in segregants with different combinations of the superior alleles, gpd1L164P, hot1P107S,H274Y and smp1R110Q,P269Q, in the selected segregant pool. The mean value of the glycerol and ethanol yield is indicated for each group. (b) Variant frequency of gpd1L164P (●), hot1P107S,H274Y (▲) and smp1R110Q,P269Q (○) in the 70 selected segregants, which were categorized according to decreasing glycerol yield and increasing ethanol yield. Glycerol yield was divided into nine bins, each with a similar number of strains, starting from <96.5 and with a bin width of 2.3%. Accordingly, ethanol yield was divided into seven bins, each with a similar number of strains, starting from <99.16 and with a bin width of 0.185%. The number of segregants in each bin is indicated in the lower panel.