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Figure 14 | Biotechnology for Biofuels

Figure 14

From: Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering

Figure 14

D-xylose isomerase activity and its effect on D-xylose fermentation capacity. (a) Specific XI activity as measured in cell extracts of strains GS1.11-26, M315 and HDY.GUF5. M315 was obtained after the EMS mutagenesis procedure of parent strain HDY.GUF5. Error bars represent the standard deviation from triplicate experiments. (b) Effect of XylA, encoding XI, overexpression from a multi-copy plasmid on D-xylose fermentation capacity. Fermentation performance in YP + 40 g/L D-xylose was estimated from weight loss due to CO2 release. The experiment was repeated with at least three independent transformants. Error bars represent standard deviations from the average of two independent transformants. Strains: (●) GS1.11-26, (○) GS1.11-26 + pXI, (▲) HDY.GUF5, (∆) HDY.GUF5 + pXI, (■) M315, (□) M315 + pXI.

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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