Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering

Table 1 Comparison of fermentation performance between the parent strain, HDY.GUF5, and the evolved strain, GS1.11-26, in YP medium containing a glucose and D-xylose mixture

Strain	Maximum specific sugar consumption rate		Yield			Specific ethanol productivity
			(g/g sugar)^a			Specific ethanol productivity
						.
	(g/g DW/h)					(g/g DW/h)
	Glucose	D-Xylose	Ethanol	Xylitol	Glycerol
HDY.GUF5	3.83 ± 0.08	0.13 ± 0.01	0.23 ± 0.00	0.03 ± 0.00	0.04 ± 0.00	1.79 ± 0.08
GS1.11-26	2.71 ± 0.04	1.10 ± 0.00	0.46 ± 0.00	0.04 ± 0.00	0.06 ± 0.00	1.38 ± 0.01

Note: ^a: The yield of ethanol and glycerol was calculated per g total sugar while yield of xylitol was calculated per g D-xylose.

ISSN: 2731-3654