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Table 3 CreA::GFP nuclear localisation post transfer between media containing different carbon sources

From: Functional characterisation of the non-essential protein kinases and phosphatases regulating Aspergillus nidulans hydrolytic enzyme production

Strain

Start culture

Nuclear CreA

Transfer culture

Nuclear CreA

CreA::GFP

Glucose 16 h (82)

100%

Xylose 2 h (48)

68%

   

Cellobiose 2 h (74)

68%

   

Xylan 5 h (114)

18%

   

AVICEL 5 h (74)

4%

   

Glycerol 2 h (32)

31%

   

No carbon 5 h (35)

0%

CreA::GFP

Xylose 16 h (24)

70%

Glucose 1 h (20)

100%

CreA::GFP

AVICEL 16 h (22)

0%

Glucose 1 h (30)

100%

CreA::GFP

Glucose 16 h followed by no carbon 5 h (50)

0%

Glucose 1 h (32)

100%

   

2-deoxyglucose 1 h (44)

100%

   

6-deoxyglucose 1 h (35)

0%

∆schA

Glucose 16 h (24)

100%

AVICEL 5 h (31)

83%

∆snfA

Glucose 16 h (19)

100%

AVICEL 5 h (28)

76%

∆atmA

Glucose 16 h (20)

100%

Avicel 5 h (22)

0%

∆xprG

Glucose 16 h (20)

100%

Avicel 5 h (20)

0%

  1. Fungal CreA::GFP nuclear localisation is presented as a percentage of the total number of hyphae evaluated (presented in brackets).