Figure 5

Determination of cohesin-dockerin specificity by affinity-based ELISA. In order to identify specific interactions between the nineteen CBM-Cohs and the four XynDocs, microtiter plates were coated with the respective CBM-Coh fusion protein, and increasing concentrations of the various XynDocs were then applied to the plates. The EC₅₀ was calculated for the resultant interactions, and values of the pEC₅₀ are presented on the y-axis in the bar graph. Coh, cohesin; Doc, dockerin; XDoc, X-dockerin modular dyad; CBM, carbohydrate-binding module. The cohesin names and numbers are shown on the horizontal axis (for example, A1 indicates the first cohesin of ScaA). Xyn-XDocA, green; Xyn-XDocH/L, dark green; XynDocB, red; XynDocGH48, yellow.