Table 1 Summary of specific gene knockout in Z. mobilis
From: Zymomonas mobilis: a novel platform for future biorefineries
Gene inactive | Method | Description | References |
|---|---|---|---|
Extracellular sucrase gene (sacC, ZMO0375) | Insertional mutant | Improves levan production | [20] |
Restriction-Modification | Insertional mutant or Homologous recombination | Increased transformation efficiency | |
(R-M) systems related gene (ZMO0028, ZMO1932, ZMO1933, ZMO1934, ZMO1935) | |||
pdc (ZMO1360) | Homologous recombination | Lower ethanol and lactate yield, and higher succinate concentration from glucose | [23] |
adhB (ZMO1596) | |||
ldhA(ZMO1237) | |||
him A (ZMO0976) | Transposon mutagenesis | Reduced himA activity and increased ethanol production compared to parental strains when cultured in a mixed-sugar medium containing xylose, especially in the presence of acetate | |
ndh (ZMO1113) | Insertional mutant | Low respiration rate, higher cell growth and ethanol yield under aerobic conditions | |
hfq (ZMO0347) | pKNOCK suicide plasmid-based mutant construction | More sensitive to multiple lignocellulosic pretreatment inhibitors and hasan increased lag phase duration and/or slower growth depending upon the conditions; and verified that hfq playsa role in tolerance to multiple biomass pretreatment inhibitors, including acetate, vanillin, furfural, and HMF | [28] |
nha A (ZMO0117) | Insertional mutant | Cell growth decreased under sodium acetate condition | [29] |
Xylose reductase (XR, ZMO0976) | Homologous recombination | Improvement of xylose utilization | [30] |
gfo (ZMO0689) | Site-specific FLP recombinase | Improves growth and ethanol production without formation of sorbitol as a by-product in sucrose medium, but yields opposite effects in high glucose | [31] |
gfo (ZMO0689) | Homologous recombination (fusion-PCR-based construction technique) | Reduction of cell growth and ethanol production under osmotic, heat and ethanol stresses | [32] |
cytC | Insertional mutant | Exhibsfilamentous shapes and reduction in growth under a shaking condition at a high temperature | [33] |
cytB (ZMO0957), ctdB (ZMO1572) | Insertional mutant | Low respiration capacity when cultivated anaerobically | [34] |
psp operon (ZMO1061-ZMO1065) | Homologous recombination (fusion-PCR-based construction technique) | Mutiple phenotypes | Our laboratory, unpublished data |
Mutant library | Transposon mutagenesis | Mutiple phenotypes | Our laboratory, unpublished data |