Figure 3
Effect of the gpdA p ::xlnR background on extracellular α-L-rhamnosidase activity. (A) xlnAp::rhaA, gpdAp::xlnR strains (AR210 and AR211) and (B) xlnBp::rhaA, gpdAp::xlnR strains (AR201 and AR206). AR137 and AR142 were respectively used as reference strains. AR137, AR210 and AR211 were grown under non-repressing, non-inducing conditions (0.1% w/v fructose) before being transferred to inducing conditions (1% w/v OSX, pH 8). AR142, AR201 and AR206 were grown in 0.1% w/v fructose before being transferred to inducing conditions (1% w/v OSX, pH 5.5). Samples were collected at the time indicated. Values (mU ml−1) are presented as means ± SD. (C) Comparison of extracellular protein levels (mg ml−1) accumulated by the xlnR engineered strains. For each strain, at least two independent cultures were carried out.