Figure 5

Representative western blot of LysA and LysA2 from experimentally infected fermentations containing transformed yeast. Fermentation samples were collected at the end of fermentation (72 h), and protein extractions were performed as described. Fermentations containing transformed yeast that were not induced served as a negative control. For analysis, 15 μL of each extract were loaded onto a 12% polyacrylamide gel. Precision Plus All Blue protein standard was used to determine relative protein size. LysA and LysA2 bands were visualized at their predicted molecular weight of 37.9 kDa and 41.1 kDa, respectively. Lane 1, fermentation containing transformed yeast expressing LysA; Lane 2, fermentation containing transformed yeast with LysA that was not induced; Lane 3, fermentation containing transformed yeast expressing LysA2; Lane 4, fermentation containing transformed yeast with LysA2 that was not induced; Lane 5, fermentation containing transformed yeast expressing LysA that was mechanically lysed; and Lane 6, fermentation containing transformed yeast expressing LysA2 that was mechanically lysed.