Predominance of Trichoderma and Penicillium in cellulolytic aerobic filamentous fungi from subtropical and tropical forests in China, and their use in finding highly efficient β-glucosidase

Table 2 Isolation and screening of fungal strains producing Avicelase activity from forests of national natural reserves in subtropical and tropical regions of China

Sample location	Samples, n	Strains isolated, n
		Culture method		Avicelase activity
		Filter paper	Avicel	> 0.05 U/ml^a	> 0.2 U/ml^b
Shankou Mangrove National Natural Reserve	31	20	5	2	2
Xishuangbanna National Natural Reserve	56	63	63	15	8
Golden Camellia National Natural Reserve	62	64	51	8	3
Shiwandashan National Natural Reserve	26	25	15	3	3
Longgang National Natural Reserve	19	14	14	1	1
Dawangling Natural Reserve	37	20	10	1	0
Huaping National Natural Reserve	37	53	53	16	9
Baimaxueshan National Natural Reserve	62	46	20	5	5
Total	330	305	231	51	31

^aIndicates that Avicelase activity was measured under conditions of pH 5.0 and 50°C 5 days after cultivation of fungal strains in liquid culture.
^bIndicates that Avicelase activity was measured under optimum pH and temperature of the crude enzyme produced by each fungal strain in liquid culture at peak enzyme production.

ISSN: 2731-3654