Figure 7
Construction of polyhydroxybutyrate (PHB) synthesis plasmid under a strong porin promoter. Plasmid pBBRMCS1 serves as backbone of expression vector pBBR1MCS-OriC-PorinP-phaCABLS. It consists of Halomonas campaniensis LS21 PHB synthesis operon including genes encoding PHA synthase phaC, β-ketothiolase phaA, NADPH-dependent acetoacetyl-CoA reductase phaB. The operon is under the control of a strong porin promoter and an oriC from the host chromosome.