Table 1 Strains and plasmids used in this study

Strains

Escherichia coli S17-1

A conjugation donor, harboring tra genes of plasmid RP4 in the chromosome; proA, thi-1

(Simon et al., 1983)

Halomonas campaniensis LS21

Halomonas campaniensis LS21 wild type, isolated from a salt lake in China

This study

Plasmids

pBBR1-MCS1

A broad-host-vector that can replicate in Halomonas TD strain, mobilizable, Cm^R

(Kovach et al., 1995)

pBBR1MCS1-OriC-Pporin-phaCAB_LS

pBBR1-MCS1 derivate expressing plasmid, an inducible vector for phaCAB over expression in Halomonas LS21 strain, Cm^R.

This study