Phylogenetic tree of pectate lyases in Arabidopsis thaliana , Populus trichocarpa , and other plants. The tree was constructed by the neighbor-joining method using MEGA5. The Populus gene numbering is consistent with  and family designation (I-V) is consistent with . The optimal tree with the sum of branch length = 5.9 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. Cryj1, Cryptomeria japonica pectate lyase [GenBank: BAA05542]; GhPel, Gossypium hirsutum pectate lyase [GenBank: ADB90478]; MaPel1, Musa acuminata pectate lyase 1 [GenBank: AAF19195]; ZePel, Zinnia elegans pectate lyase [GenBank: Y09541].