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Table 1 Lignin, hydrolyzable sugar anhydrate content and crystalline cellulose content of aspen wood

From: Aspen pectate lyase Ptxt PL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

 

Wild-type

1051

1002

Klason lignin

16.8 ± 0.2 1 *

17.7 ± 0.3**

17.1 ± 0.3

Acid soluble lignin

2.1 ± 0.0

2.1 ± 0.0

2.0 ± 0.1

Cellulose

364 ± 28

346 ± 62

363 ± 28

Rha

11.7 ± 1.2

9.2 ± 0.6

9.0 ± 1.2

Ara

5.6 ± 0.1

6.8 ± 0.4

6.1 ± 0.5

Xyl

312.4 ± 5.7

304.1 ± 6.4

317.8 ± 2.1

Man

20.5 ± 0.4**

18.8 ± 0.6

16.1 ± 0.9**

Gal

11.5 ± 0.9

13.3 ± 0.5

13.1 ± 1.0

Glc

178.8 ± 0.4*

187.2 ± 2.3

191.9 ± 4.8

Total sugar

540.4 ± 7.4

539.4 ± 5.3

554.1 ± 6.8

Uronic acids

31.8 ± 2.4

30.4 ± 2.0

27.9 ± 1.5

  1. Lignin content is expressed in % (w/w). Sugar content is expressed in μg/mg of wood de-starched alcohol-insoluble residue (AIR). Means ± standard error, n = 10 or 2 biological replicates for lignin and sugars, respectively.
  2. 1Averages for the transgenic lines in bold are significantly different from wild-type (WT) (post analysis-of-variance (ANOVA) t-test). Averages for WT in bold are significantly different from both transgenic lines taken together (post-ANOVA contrast). *P ≤10%; **P ≤5%.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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