Aspen pectate lyase Ptxt PL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield

Table 2 Total sugar yields for transgenic aspen lines and wild-type after acidic pretreatment

Line	Total sugar yields (pretreatment liquid and enzymatic hydrolysate)
Line	Y_Hexoses/W(g g^-1)^a	Y_Pentoses/W(g g^-1)^b	Y_Total/Wv(g g^-1)^c
Wild-type	0.390 ± 0.01 (100%)**	0.126 ± 0.01 (100%)***	0.517 ± 0.01 (100%)***
1051	0.421 ± 0.01 (108%)**	0.144 ± 0.01 (114%)**	0.565 ± 0.01 (109%)**
1002	0.418 ± 0.03 (107%)	0.157 ± 0.01 (124%)**	0.574 ± 0.01 (111%)**

The % values represent the sugar yield in comparison with wild-type (WT). Means ± standard error, n = 5 biological replicates. ^aYield of hexoses (g of Gal, Glc, and Man) per g of wood after pretreatment and after 72 h of enzymatic hydrolysis. ^bYield of pentoses (g of Ara and Xyl) per g of wood after pretreatment and after 72 h of enzymatic hydrolysis. ^cYield of monosaccharides (g of Ara, Gal, Glc, Man and Xyl) per g of wood after pretreatment and after 72 h of enzymatic hydrolysis. Asterisks (by means for individual lines) indicate yields significantly different from WT when analyzed by post analysis-of-variance (ANOVA) t-test or means (for WT) that were significantly different from both lines by post ANOVA contrast. **P ≤5%, ***P ≤1%.

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